-
Notifications
You must be signed in to change notification settings - Fork 11
Commit
This commit does not belong to any branch on this repository, and may belong to a fork outside of the repository.
- Loading branch information
j23414
committed
Mar 25, 2023
1 parent
b2a6cfa
commit 645acb2
Showing
39 changed files
with
2,461 additions
and
0 deletions.
There are no files selected for viewing
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,87 @@ | ||
# nextstrain.org/monkeypox/ingest | ||
|
||
This is the ingest pipeline for Monkeypox virus sequences. | ||
|
||
## Usage | ||
|
||
> NOTE: All command examples assume you are within the `ingest` directory. | ||
> If running commands from the outer `monkeypox` directory, please replace the `.` with `ingest` | ||
Fetch sequences with | ||
|
||
```sh | ||
nextstrain build --cpus 1 . data/sequences.ndjson | ||
``` | ||
|
||
Run the complete ingest pipeline with | ||
|
||
```sh | ||
nextstrain build --cpus 1 . | ||
``` | ||
|
||
This will produce two files (within the `ingest` directory): | ||
|
||
- data/metadata.tsv | ||
- data/sequences.fasta | ||
|
||
Run the complete ingest pipeline and upload results to AWS S3 with | ||
|
||
```sh | ||
nextstrain build . --configfiles config/config.yaml config/optional.yaml | ||
``` | ||
|
||
### Adding new sequences not from GenBank | ||
|
||
#### Static Files | ||
|
||
Do the following to include sequences from static FASTA files. | ||
|
||
1. Convert the FASTA files to NDJSON files with: | ||
|
||
```sh | ||
./ingest/bin/fasta-to-ndjson \ | ||
--fasta {path-to-fasta-file} \ | ||
--fields {fasta-header-field-names} \ | ||
--separator {field-separator-in-header} \ | ||
--exclude {fields-to-exclude-in-output} \ | ||
> ingest/data/{file-name}.ndjson | ||
``` | ||
|
||
2. Add the following to the `.gitignore` to allow the file to be included in the repo: | ||
|
||
```gitignore | ||
!ingest/data/{file-name}.ndjson | ||
``` | ||
|
||
3. Add the `file-name` (without the `.ndjson` extension) as a source to `ingest/config/config.yaml`. This will tell the ingest pipeline to concatenate the records to the GenBank sequences and run them through the same transform pipeline. | ||
|
||
## Configuration | ||
|
||
Configuration takes place in `config/config.yaml` by default. | ||
Optional configs for uploading files and Slack notifications are in `config/optional.yaml`. | ||
|
||
### Environment Variables | ||
|
||
The complete ingest pipeline with AWS S3 uploads and Slack notifications uses the following environment variables: | ||
|
||
#### Required | ||
|
||
- `AWS_DEFAULT_REGION` | ||
- `AWS_ACCESS_KEY_ID` | ||
- `AWS_SECRET_ACCESS_KEY` | ||
- `SLACK_TOKEN` | ||
- `SLACK_CHANNELS` | ||
|
||
#### Optional | ||
|
||
These are optional environment variables used in our automated pipeline for providing detailed Slack notifications. | ||
|
||
- `GITHUB_RUN_ID` - provided via [`github.run_id` in a GitHub Action workflow](https://docs.github.com/en/actions/learn-github-actions/contexts#github-context) | ||
- `AWS_BATCH_JOB_ID` - provided via [AWS Batch Job environment variables](https://docs.aws.amazon.com/batch/latest/userguide/job_env_vars.html) | ||
|
||
## Input data | ||
|
||
### GenBank data | ||
|
||
GenBank sequences and metadata are fetched via NCBI Virus. | ||
The exact URL used to fetch data is constructed in `bin/genbank-url`. |
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,82 @@ | ||
if not config: | ||
|
||
configfile: "config/config.yaml" | ||
|
||
|
||
send_slack_notifications = config.get("send_slack_notifications", False) | ||
|
||
|
||
def _get_all_targets(wildcards): | ||
# Default targets are the metadata TSV and sequences FASTA files | ||
all_targets = ["data/sequences.fasta", "data/metadata.tsv"] | ||
|
||
# Add additional targets based on upload config | ||
upload_config = config.get("upload", {}) | ||
|
||
for target, params in upload_config.items(): | ||
files_to_upload = params.get("files_to_upload", []) | ||
remote_file_names = params.get("remote_file_names", []) | ||
|
||
if len(files_to_upload) != len(remote_file_names): | ||
print( | ||
f"Skipping file upload for {target!r} because the number of", | ||
"files to upload does not match the number of remote file names.", | ||
) | ||
elif len(remote_file_names) != len(set(remote_file_names)): | ||
print( | ||
f"Skipping file upload for {target!r} because there are duplicate remote file names." | ||
) | ||
elif not params.get("dst"): | ||
print( | ||
f"Skipping file upload for {target!r} because the destintion was not defined." | ||
) | ||
else: | ||
all_targets.extend( | ||
expand( | ||
[ | ||
f"data/upload/{target}/{{file_to_upload}}-to-{{remote_file_name}}.done" | ||
], | ||
zip, | ||
file_to_upload=files_to_upload, | ||
remote_file_name=remote_file_names, | ||
) | ||
) | ||
|
||
# Add additional targets for Nextstrain's internal Slack notifications | ||
if send_slack_notifications: | ||
all_targets.extend( | ||
[ | ||
"data/notify/genbank-record-change.done", | ||
"data/notify/metadata-diff.done", | ||
] | ||
) | ||
|
||
if config.get("trigger_rebuild"): | ||
all_targets.append("data/trigger/rebuild.done") | ||
|
||
return all_targets | ||
|
||
|
||
rule all: | ||
input: | ||
_get_all_targets, | ||
|
||
|
||
include: "workflow/snakemake_rules/fetch_sequences.smk" | ||
include: "workflow/snakemake_rules/transform.smk" | ||
include: "workflow/snakemake_rules/nextclade.smk" | ||
|
||
|
||
if config.get("upload"): | ||
|
||
include: "workflow/snakemake_rules/upload.smk" | ||
|
||
|
||
if send_slack_notifications: | ||
|
||
include: "workflow/snakemake_rules/slack_notifications.smk" | ||
|
||
|
||
if config.get("trigger_rebuild"): | ||
|
||
include: "workflow/snakemake_rules/trigger_rebuild.smk" |
Oops, something went wrong.