Add Dengue virus All serotypes dataset

data/nextstrain/collection.json

@@ -53,6 +53,7 @@
     "nextstrain/flu/h1n1pdm/pb1",
     "nextstrain/flu/h3n2/pb2",
     "nextstrain/measles",
-    "nextstrain/measles/N450/WHO-2012"
+    "nextstrain/measles/N450/WHO-2012",
+    "nextstrain/dengue/all"
   ]
 }

data/nextstrain/dengue/all/CHANGELOG.md

@@ -0,0 +1,5 @@
+## Unreleased
+
+Initial release for Nextclade v3!
+
+Read more about Nextclade datasets in the documentation: https://docs.nextstrain.org/projects/nextclade/en/stable/user/datasets.html

data/nextstrain/dengue/all/README.md

@@ -0,0 +1,26 @@
+# Dengue dataset
+
+| Key  | Value  |
+| :-- | :-- |
+| name  | Dengue virus All serotypes |
+| authors | [Nextstrain](https://nextstrain.org) |
+| reference | NC_002640.1 |
+| workflow  | https://github.com/nextstrain/dengue/tree/main/nextclade  |
+| path  | `nextstrain/dengue/all` |
+
+
+## Scope of this dataset
+
+This dataset assigns serotype (e.g. DENV1-DENV4) to dengue samples based on [criteria outlined by the WHO](https://pubmed.ncbi.nlm.nih.gov/26868382/) and tree placement nearest references [NC_001477.1 (DENV1)](https://www.ncbi.nlm.nih.gov/nuccore/NC_001477.1), [NC_001474.2 (DENV2)](https://www.ncbi.nlm.nih.gov/nuccore/NC_001474.2), [NC_001475.2 (DENV3)](https://www.ncbi.nlm.nih.gov/nuccore/NC_001475.2), and [NC_002640.1 (DENV4)](https://www.ncbi.nlm.nih.gov/nuccore/NC_002640.1).
+
+## Features
+
+This dataset supports:
+
+- Assignment of serotypes
+- Phylogenetic placement
+- Sequence quality control (QC)
+
+## What are Nextclade datasets
+
+Read more about Nextclade datasets in the Nextclade documentation: https://docs.nextstrain.org/projects/nextclade/en/stable/user/datasets.html

data/nextstrain/dengue/all/genome_annotation.gff3

@@ -0,0 +1,14 @@
+##gff-version 3
+##sequence-region Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome 1 10649
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome	feature	gene	102	440	.	+	.	codon_start=1;gene=C;gene_name=C;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome	feature	gene	441	713	.	+	.	codon_start=1;gene=pr;gene_name=pr;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome	feature	gene	441	938	.	+	.	codon_start=1;gene=M;gene_name=M;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome	feature	gene	939	2423	.	+	.	codon_start=1;gene=E;gene_name=E;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome	feature	gene	2424	3479	.	+	.	codon_start=1;gene=NS1;gene_name=NS1;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome	feature	gene	3480	4133	.	+	.	codon_start=1;gene=NS2A;gene_name=NS2A;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome	feature	gene	4134	4523	.	+	.	codon_start=1;gene=NS2B;gene_name=NS2B;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome	feature	gene	4524	6377	.	+	.	codon_start=1;gene=NS3;gene_name=NS3;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome	feature	gene	6378	6758	.	+	.	codon_start=1;gene=NS4A;gene_name=NS4A;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome	feature	gene	6759	6827	.	+	.	codon_start=1;gene=2K;gene_name=2K;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome	feature	gene	6828	7562	.	+	.	codon_start=1;gene=NS4B;gene_name=NS4B;
+Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome	feature	gene	7563	10262	.	+	.	codon_start=1;gene=NS5;gene_name=NS5;

data/nextstrain/dengue/all/pathogen.json

@@ -0,0 +1,71 @@
+{
+  "alignmentParams": {
+    "penaltyGapOpen": 8,
+    "penaltyGapOpenInFrame": 12,
+    "penaltyGapOpenOutOfFrame": 14,
+    "gapAlignmentSide": "left",
+    "minSeedCover": 0.01,
+    "minLength": 1000
+  },
+  "attributes": {
+    "name": "Dengue virus All serotypes",
+    "reference accession": "NC_002640",
+    "reference name": "dengue virus type 4"
+  },
+  "compatibility": {
+    "cli": "3.0.0-alpha.0",
+    "web": "3.0.0-alpha.0"
+  },
+  "deprecated": false,
+  "enabled": true,
+  "experimental": true,
+  "files": {
+    "changelog": "CHANGELOG.md",
+    "examples": "sequences.fasta",
+    "genomeAnnotation": "genome_annotation.gff3",
+    "pathogenJson": "pathogen.json",
+    "readme": "README.md",
+    "reference": "reference.fasta",
+    "treeJson": "tree.json"
+  },
+  "meta": {
+    "bugs": "https://github.com/nextstrain/nextclade_data/issues",
+    "source code": "https://github.com/nextstrain/nextclade_data"
+  },
+  "qc": {
+    "frameShifts": {
+      "enabled": false
+    },
+    "missingData": {
+      "enabled": false,
+      "missingDataThreshold": 2700,
+      "scoreBias": 300
+    },
+    "mixedSites": {
+      "enabled": false,
+      "mixedSitesThreshold": 10
+    },
+    "privateMutations": {
+      "cutoff": 24,
+      "enabled": false,
+      "typical": 8,
+      "weightLabeledSubstitutions": 2,
+      "weightReversionSubstitutions": 1,
+      "weightUnlabeledSubstitutions": 1
+    },
+    "snpClusters": {
+      "clusterCutOff": 5,
+      "enabled": false,
+      "scoreWeight": 50,
+      "windowSize": 100
+    },
+    "stopCodons": {
+      "enabled": true
+    }
+  },
+  "schemaVersion": "3.0.0",
+  "version": {
+    "tag": "unreleased"
+  },
+  "defaultCds": "E"
+}

data/nextstrain/dengue/all/reference.fasta

@@ -0,0 +1,2 @@
+>Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome
+AGTTGTTAGTCTACGTGGACCGACAAGGACAGTTTCGAATCGGAAGCTTGCTTAACGTAGTTCTAACAGTTTTTTTAATTAGAGAGCAGATCTCTGAGATGAACAACCAACGGAAAAAGGCGGGAAAACCGCCTTTCAATATGCTGAAACGCGAGAGAAACCGCGTGTCAACTGTACAACAGTTGGCGAAGAGATTCTCAATAGGATTGCTGTAAGGGCAAGGACCATTAAAATTGGTTATGGCTTTCATAGCATTCCTACGATTCCTAGCCATCCCACCAACAGCAGGGATTTTGAAAAGATGGGGAACATTCAAGAAGTCTAAGGCTATCAAGGTACTGAGAGGATTCAGGAAGGAGATCGGAAGCATGCTGAACATTTTGAACAGGAGAAAAAGAACAGCAATCATTCTCATGATGCTGATTCCAACAGTAATGGCGTTCCATTTGACCACACGAGATGGAGAGCCGCACATGATTGTGAGCAAGCATGAAAGAGGAAAATCCCTTCTGTTTAAGACAGCGGATGGAATCAACATGTGCACCCTCATTGCCATGGATCTGGGTGAGTTGTGTGAGGACACAATCACGTACAAATGCCCCCTTCTCACCGAAACCGAACCAGAAGACATTGACTGCTGGTGCAATCCCACGTCCACATGGGTGACCTATGGGACGTGTACCCAAACTGGAGAACACAGACGAGAGAAAAGATCAGTGGCACTAGCCCCACATGTGGGAATGGGACTGGAAACAAGAACTGAAACATGGATGTCATCAGAAGGAGCTTGGAAACAAGCCCAGAGAGTAGAGACTTGGATTCTGAGACACCCAGGATTCACGATAATGGCAGTATTTCTGGCATACACCATAGGAACAACATATATCCAGAGAGTCGTTATTTTTATCCTACTGATGCTGGTCGCCCCATCCATGACAATGCGATGCGTGGGAGTAGGAAACAGAGACTTTGTGGAAGGAGTTTCAGGAGGAACGTGGGTTGACGTGGTGCTAGAACATGGAAGTTGTGTCACAACCATGGCAAAGAACAAACCAACACTGGACTTTGAACTGATGAAGACAGAAGCCAAACAACCGGCCACACTAAGGAAATTCTGCATTGAAGCTAAAATAACCAACACAACAACAGAATCAAGATGTCCAACACAAGGGGAACCCAATCTAGTAGAGGAGCAGGACCAGAAGTTCGTCTGCAAACAAACGATGGTGGACAGAGGATGGGGCAATGGCTGTGGTTTGTTTGGAAAAGGAGGCATTGTGACATGTGCGAAGTTTACATGCTTGAAGAAAATAGAAGGAAAAGTGGTGCAACCTGAAAACTTAGAATACACCGTGGTCATAACAGTCCACACAGGAGACGAACACGCAGTGGGAAATGACACAACAAAACATGGAATGACGGCCAAGATAACACCCCAGGCTTCCACCGCAGAAGCTAAACTGACAGACTATGGAACTCTCACGCTGGATTGCTCACCAAGAACAGGTCTCGACTTTAATGAGATGGTCTTGTTGAAAATGAAAAACAAAGCATGGCTTGTACACAGACAATGGTTCCTAGACCTACCTCTACCATGGACATCAGGAGCTGACACATAAGAAGCAAATTGGAACCAGAAAGAGATGCTGGTGACATTCAAAAATCCTCATGCAAAGAAACAGGATGTAGTCGTCCTAGGATCCCAAGAAGGAGCCATGCACACAGCACTCACAGGAGCTACAGAAATCCAAACGTCAGGAGGAAACTTAATGTTTGCAGGACACCTGAAGTGCAGACTGAGAATGGACAAACTGAAACTCAAAGGGATGTCATACGCGATGTGCACAGGAAAGTTCAAAATTGAGAAAGAAATGGCAGAAACACAGCATGGAACAATAGTCATTAAGGTTAAGTATGAAGGAGATGGTGCACCATGCAAGATCCCTTTTGAGATAAAGGATGTGAAAAAGAAAAATGTCATTGGGCGACTGATCACAGCCAACCCAATAGTCACAGAGAAAGACAGTCCAGTCAACATTGAAGCAGAACCTCCTTTTGGGGACAGCTACATCGTGATAGGAGTAGGAGAAAGAGCATTGAAACTCAACTGGTTCAAGAAAGGAAGCTCCATTGGGAAAATGTTTGAGGCAACTGCCAGAGGAGCAAAAAGAATGGCCATCTTGGGAGACACAGCCTGGGATTTTGGATCTGTAGGAGGAGTGTTCACATCAGTAGGAAAGGCGGTGCACCAGGTTTTTGGAACTGTCTATGGGGCTATGTTTAGCGGAGTCTCATGGACCATGAAAATCCTAATAGGGGTTCTCATAACATGGATAGGAATGAATTCAAGAAGCACTTCCCTGTCAATGACATGCATAGCAGTAGGAATCGTCACACTGTACCTGGGAGTCATGGTGCAGGCGGACATGGGTTGTGTTGTGAACTGGAAAGGCAAAGAACTGAAATGTGGAAGTGGAATTTTTGTCACTGACAACGTGCACACTTGGACAGAACAATACAAATTCCAACCAGACTCCCCAACAAGACTGGCGTCAGCCATTCCGAAAGCTCATGAGGAGGGAGTCTGTGGAATCCGATCAGCAACAAGACTGGAGAATCTCATGTGGAAGCAAATAACAAATGAATTGAACCACATCCTATGGGAAAATGAAATCAAACTAACTGTAGTGGCAGGAGACATTAAAGGAATCTTGGAGAAAGGGAAAAGAACACTTACACCTCAACCCATTGAGCTGAAATATTCATGGAAAACATGGGGAAAAGCAAAAATACTCACAGCAGAAGTACAAAATAACACCTTCCTCATAGACGGTCCAGACACACCAGAATGCCCTAATGACCAAAGAGCATGGAATTTTCTGGAAGTTGAGGACTATGGATTTGGAATCTTCACGACCAACATATGGCTGAAATTGCGAGAAGAGTACACCGAAGTGTGTGACCACAGGCTAATGTCAGCAGCCATCAAGGACAACAGGGCCGTCCATGCTGACATGGGCTATTGGATAGAAAGCGCAAAGAATGAGACCTGGAAGATAGAGAAAGCATCCTTCATTGAAGTTAAAACCTGCCTCTGGCCAAAATCACACACTCTTTGGAGCAATGGAGTGCTAGAAAGTGAAATGATAATTCCAAAGATTTTTGCTGGACCAATTTCACAGCACAACTACAGACCAGGCTATTACACACAAACAGCAGGACCATGGCACTTAGGCAAATTGGAGATGGACTTTGATTTTTGTGAAGGAACCACAGTTGTAGTGACTGAGGACTGTGGAAATAGAGGCCCATCTTTGAGAACAACCACAGCCTCAGGAAAACTGATAACTGAATGGTGCTGCCGATCTTGCACATTACCTCCCCTAAGATACATAGGAGAGGATGGATGCTGGTATGGGATGGAAATCAGACCCTTGAATGAGAAAGAAGAGAACCTGGTCAAGTCATTGGTCACAGCCGGGCATGGAAAGGTGGACAATTTTTCAATAGGACTCTTGTGCATGGCATTGTTCATGGAAGAGGTGCTGAGAACCAGAGTTGGCAGAAAACACATGATATTAGTAGTAGTGGTTACCTTCGTGACCCTCATCACAGGGAATATGACATGGAGAGACCTGGTAAGAGTACTTATCATGGTGGGAGCCACTATGTCTGATAGAATGGGGGGCGTGACTTACCTAGCTTTAATGGCAGCTTTCAAAATGAGACCAATCTTCGCAGTGGGATTGTTCTTGAGGAAACTGACTTCAAGAGAAATAATACTGATGACAATAGGAATGGCCCTGGTAACATCGTTCCAAATACCAGATGCCATTGTGGAACTGACTGATGGAATAGCATTGGGGATAATGGTGCTTAAAATGGTGACACATTTTGAAAAATACCAGTTGGGAGCGACCATCCTGTCCTTGACGTGCATCAAAACAACTATGACATTGCATAATGCATGGAAAACAATCACCATTATATTGGCAGTAGTTTCTCTTTTCCCATTGTGCCTGACTTCCTCCCTTCAAAAACAGACAGATTGGATACCAATAACATTGACAATCATAGGAGTCAAACCACTACCCATGTTTCTAATAACTTTAACAAAAACCAGCAAAAAAAGAAGTTGGCCTCTCAATGAGGGCATTATGGCTGTTGGAATGGTGAGCATTCTAGCAAGTTCTCTCCTAAAGAATGATGTTCCCATGGCTGGACCATTAGTGGCTGGAGGCTTGCTCATAGCGTGCTACGTGATAACTGGCAGCTCAGCAGACTTGTCACTGGAGAAAGCCGCCGATGTGAAATGGGAAGAAGAGGCAGAGATATCAGGAGCCAGCCCAATCATATTAGTCACAGTAGAAGAAGATGGTACCATGTCAATAAAGGATGAAGAGGAAGAGAACACACTAACCATACTCATAAAAACAGCACTGCTAGTAGTGTCAGGCCTCTTCCCATTGTCAATACCAGTCACAATGGCGGTATGGTACATGTGGCAAATGAAAAAACAAAGATCCGGAGTGCTGTGGGACGTCCCCTCACCCCCAGCAGTGGAAAAAGCTGAACTGGAAGATGGAGTCTATAGAATCAAGCAAAGAGGGATTTTTGGGAAATCCCAAGTTGGAGTTGGAGTATACAAAGAAGGAGTATTCCACACAATGTGGCACGTCACAAGAGGAGCTGTCCTCATGCATAAAGGGAAGAGACTGGAACCATCTTGGGCTGACGTCAAGAAAGACTTGATATCATATGGAGGAGGTTGGAGGCTTGAAGGAAAATGGAAAAAAGGAGAAGAAGTGCAGGTCTTGGCCGTAGAACCTGGGAAAAATCCAAAAAACGTCCAAACAAAACCTGGCCTTTTCAAGACCACTACAGGAGAAATAGGAGCCGTAGCCCTGGACTTCAAACCTGGAACATCAGGATCTCCCATCATTAACAAAAAAGGAAAAGTTGTGGGTCTCTATGGAAATGGAGTGGTTACAAAAAGTGGAGCCTATGTCAGTGCCATAGCGCAAACTGAAAAAATTGAAGAGCCGAATCCAGAGATTGAAGATGACATCTTTAGAAAGAAAAGATTAACCATAATGGACCTACATCCAGGAGCGGGAAAGACAAAAAGATATCTCCCAGCCATAGTCAGAGAGGCCATAAAAAGAAGCCTGAGAACTCTAATCTTGGCTCCCACAAGAGTGGTGGCAGCTGAAATGGAAGAAGCCCTAAGAGGACTCCCAATAAGTTATCAGACCCCAGCCATGAAGGCTGAACACACAGGAAGGGAGATTGTAGACCTAATGTGCCATGCCACTTTCACCATGAGACTGCTGTCACCAATCAGGGTTCCAAATTACAACCTGATAATAATGGATGAAGCACATTTCACAGACCCAGCCAGCATAGCGGCTAGAGGATACATCTCAACCCGAGTGGAAATGGGAGAGGCAGCTGCAATTTTTATGACAGCCACTCCCCCAGGAACGACAGATCCCTTTCCTCAGAGCAACGCCCCAATCAAAGATGAAGAAAGAGAAATTCCTGAACGCTCATGGAATTCAGGATATGAATGGATCACAGATTTTCAAGGGAAGACAGTGTGGTTTGTTCCAAGCATAAAAGCCGGAAATGACATAGCAAACTGCTTGAGAAAAAATGGAAAAAGGGTGATTCAACTGAGTAGGAAAACCTTTGACACAGAATATCCAAAAACAAAAATCAATGACTGGGACTTTGTGGTCACAACAGACATTTCCGAAATGGGAGCCAATTTCAAAGCTGAGAGAGTGATAGACCCAAGGAGATGCCTGAAGCCAGTTATACTGACAGATGGTCCAGAGCGGGTGATTCTGGCAGGACCCATGCCAGTGACCCTAGCAAGTGCTGCCCAAAGAAGAGGGAGAATAGGAAGGAACCCAAAAAAAGAAAATGACCAGTACATTTACATGGGAGAGCCTCTAAAAAATGATGAAGACCATGCTCACTGGACAGAAGCAAAGATGCTCCTTGACAACATCAACACACCAGAAGGGATCATCCCAACCCTGTTTGAACCAGAAAGGGAAAAGACTGAAGCCATAGACGGTGAATACCGCCTGAGAGGGGAAGCAAGGAAAACCTTTGTGGAACTAATGAGAAGAGGAGACCTACCAGTTTGGCTGGCCTACAAAGTAGCTTCAGAAGGCATCAAATACACAGACAGAAGGTGGTGCTTTGATGGAGAAAGGAACAATCAAATTTTGGAGGAGAACATGGAAGTGGAAATCTGGACAAAAGAAGGAGAAAGGAAGAAATTAAGACCTAGATGGTTGGATGCTAGAACTTATTCTGACCCACTGGCACTAAAGGAATTCAAGGAGTTTGCAGCTGGAAGAAAGTCCATAACCCTTGACCTAATAACAGAAATAGGTAGACTGCCAACTTACTTGACCCACAGGGCCAGAAATGCCCTGGACAACTTGGTCATGCTGCACACGACAGAAAAAGGAGGAAGGGCCTACAAACATGCTCTGAATGAACTACCAGAAACCCTGGAAACATTCATGCTCCTAGCTCTGTTGGCTGCGATGACAGGAGGAATCTTTCTGTTCTTGATGTAAGGAAGAGGTATAGGGAAAATGTCCATGGGACTGATCTGCATAATGGCAGCCAGCGGCCTGCTATGGATGGCAGAAATCCAGCCCCATTGGATAGCGGCCTCAATAATACTGGAGTTTTTTCTCATGGTACTGCTTATTCCAGAACCAGAAAAGCAGAGAACTCCCCAGGACAACCAATTGACCTATGTCGTGATAGCCATCCTCACAATGGTTGCAACAATAGCAGCCAATGAAATGGGATTGTTGGAAAAAACAAAGAAAGATCTTGGGTTTGGCCATGTAGCAACTGAAAATACAATTCTGGACGTAGATCTACGTCCAGCATCAGCATGGACACTCTATGCAGTGGCCACAACAATTATCACACCAATGTTGAGACACACCATAGAAAACTCCTCAGCAAATGTGTCCCTGACAGCCATTGCCAACCAGGCAGCGGTCTTGATGGGTCTTGGAAAAGGATGGCCATTATCGAAAATGGACATAGGAGTTCCACTTCTCGCCTTGGGATGCTATTCACAAGTGAACCCAATAACTCTCACAGCAGCACTACTTTTGCTAGTGGCACATTATGCCATAATAGGTCCAGGACTGCAAGCAAAAGCCACTAGAGAAGCTCAGAAAAGGACAGCAGCTGGAATCATGAAGAATCCAACGGTGGATGGGATAACAGTAATAGACCTAGATCCAATACCCTATGATCCAAAATTTGAAAAGCAGTTAGGACAAGTAATGCTCCTGGTCCTTTGTGCAGCACAGCTACTGTTGATGAGAACTACATGGGCTTTGTGTGAAGCCTTAACCCTAGCCACAGGACCAATATCAACACTGTGGGAAGGAAATCCAGGGAAATTCTGGAACACAACGATAGCTGTGTCCATGGCTAACATTTTCAGAGGGAGTTATTTGGCCGGAGCTGGACTTGCTTTTTCTATCATGAAGAATGCAGGAAGCGCAAGGAGAGGAACTGGGACCATAGGAGAAACACTGGGAGAAAAATGGAAAAGACAACTAAACCCATTGGGCAGGAAAGAATTCGAAATATACAAGAAAAGTGGAATTCTAGAAGTGGACAGAACCGAAGCCAAAGAAGGCTTGAAAAGAGGAGAAACAACCCACCATGCCGTGTCGAGAGGCTCCGCAAAACTGAGATGGTTCGTTGAGAGAAACATGGTCAAACCAGAAGGGAAAGTCGTAGACCTTGGTTGTGGAAGAGGAGGCTGGTCATATTACTGTGCGGGACTGAAGAATGTGACAGAAGTGAAAGGATACACAAAAGGAGGACCAGGACATGAAGAACCAATCCCTATGTCTACATATGGATGGAACCTAGTCAAGCTTCAGAGTGGAGTGGATGTGTTCTTCACTCCACCTGAGAAATGTGACACCCTGTTGTGTGACATTGGGGAATCATCACCAAATCCAACAGTAGAAGAAGGAAGAACATTAAGAGTTCTGAAGATGGTGGAACCATGGCTCAACAACAACACTCAATTTTGCATAAAAGTTCTCAACCCCTACATGCCAACAGTCATAGAAAAACTGGAAAAACTACAAAGGAAACATGGAGGAATCCTTGTGAGAAATCCACTCTCACGAAATTCCACCCATGAGATGTACTGGGTGTCCAATGCTTCAGGAAACATTGTGTCTTCAGTGAACATGATATCAAGAATGTTGCTGAACAGATTCACAATGAGACACAGGAAACCCACTTATGAGAAAGACGTGGACCTTGGAGCTGGAACACGAAATGTCGCAATTGAAACAGAAACACCAAACATGGATATCATTGGGAAAAGGATAGAGAAAATAAAAGAAGAGCATAAAACAACATGGCACTATGATCAGGACAATCCATACAAAACATGGGCTTACCATGGAAGCTATGAAGCCAAACCGACAGGATCAGCATCATCCATGGTGAATGGAGTGGTGAGACTGCTGACAAAACCCTGGGACGTGATTCCCATGGTGACACAGATGGCAATGACAGACACAACTCCCTTTGGACAACAGAGAGTTTTTAAAGAGAAAGTGGACACGAGAACACCAAAACCAAAACCAGGCACAAGAAAAGTTATGAAGATCACAGCCGAGTGGCTTTGGAGATTCCTTGGAAGGAAAAAAAAACCCAGAATGTGCACAAGAGAAGAGTTCACAAAAAAAGTTAGAACCAACGCAGCTATGGGCGCCGTGTTCACTGATGAGAACAAATGGAACTCAGCAAGTGAAGCTGTTGAAGACGAACGGTTTTGGGAACTGGTGGACAAAGAAAGGGATCTTCACCAAGAAGGAAAGTGTGAAACGTGTGTCTACAACATGATGGGAAAAAGAGAGAAGAAACTAGGAGAGTTTGGCAAAGCAAAAGGCAGCAGAGCAATATGGTACATGTGGCTGGGAGCACGCTTCCTAGAGTTTGAAGCCCTAGGTTTCTTGAATGAAGACCACTGGTTCTCCAGAGAAAATTCACTGAGTGGAGTGGAAGGAGAAGGACTGCACAAGCTGGGATACATCCTAAGAGACATAAACAAGAAAGAAGGAGGAGCAATGTATGCTGATGACACAGCTGGTTGGGACACAAGAATCACAGAAGATGACCTGCAGAATGAAGAAATGATCACAGACCAAATGGACCCTGAACATAAGAAATTGGCCAAGGCCATTTTCAAACTAACATACCAAAACAAGGTGGTGAGAGTCCAAAGACCAACACCAAAAGGCACGGTGATGGATATCATATCCAGGAGAGACCAAAGAGGCAGTGGACAGGTTGGAACTTATGGTCTGAACACTTTCACCAACATGGAAGCACAACTAATCAGACAAATGGAAGGTGAAGGAGTCTTTACACCAAAAGAATTCTAGACCCCACATCTGCTAGAAAAAAGAGTTCAAGAATGGTTGGAAAAACATGGAGTCGAAAGGTTAAAAAGAATGGCAATCAGTGGAGATGATTGTGTGGTGAAACCACTAGATGACAGGTTCGCCACAGCCCTAACAGCTTTGAATGACATGGGAAAGGTTAGGAAAGACATACCGCAATGGGAACCTTCAAAAGGATGGAATGATTGGCAACAAGTGCCTTTCTGCTCCCACCACTTTCATGAGCTGATCATGAAAGATGGTCGCACGCTAGTAGTTCCATGCAGAAACCAAGATGAACTGATAGGTAGAGCCAGAATCTCTCAGGGAGCTGGATGGAGCTTGAGAGAAACAGCCTGTCTGGGGAAGTCTTACGCCCAAATGTGGAGGCTGATGTACTTCCACAGAAGAGATCTGAGGCTGGCGGCCAATGCCATATGTTCAGCAGTTCCAGTACATTGGGTTCCAACAAGCAGAACAACCTGGTCGATCCATGCCCACCATCAATGGATGACAACAGAAGACATGTTTACAGTCTGGAACAGGGTGTGGATAGAGGACAACCCATGGATGGAAGACAAAACTCCAGTCTAATCTTGGGAAGACATACCATACCTAGGGAAAAGAGAAGACCAGTGGTGTGGATCCCTGATTGGCCTAACATCCAGGGCCACCTGGGCAAAGAACATTCAAACAGCCATAAACCAAGTCAGAAGCCTCATTGGGAATGAGGAGTATATAGATTACATGCCATCAATGAAAAGATTCAGGAATGAGGAGGAGAACGAAGGAGTTCTGTGGTAAACAGCAAAAGAAGTCAGGCATAAAAGAAGTCAGGCCACTAATGCCACGGCTTGAGCAAACCGTGCTGCCTGTAGCTCCACCAAAAAGGGGAGGTGTAAAAAAACCCCGGGAGGCCACAAACCATGGAAGCTGTACGCATGGTGTAGTGGACTAGCGGTTAGAGGAGACCCCTCCCATGACAGACAAAACGCAGCACAAGGGGGCCCAAAGCCAGGAGGAAGCTGTACTCTCGTTGGAAGGACTAGAGGTTAGAGGAGACCCCCCCAAAACAAAAACAGCATATTGACGCTGGGAAAGACCAGAGATCCTGCTGTCTCCTCAGCATCATTCCAGGCACAGAACGCCAGAAAATGGAATGGTGCTGTTGAATCAACAGGTTCT