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Release pixelator 1.3.0 #106

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Jul 17, 2024
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a13c0df
chore: bump to 1.3.0dev
fbdtemme May 29, 2024
559d95a
Add new changelog entry for dev version
fbdtemme May 29, 2024
2252db1
Merge pull request #95 from nf-core/sync-dev-after-1.2.0
fbdtemme May 29, 2024
56da1fd
Make all ext.args assignments closures
fbdtemme Jun 14, 2024
ccb35d0
Update CHANGELOG
fbdtemme Jun 14, 2024
27c8743
Merge pull request #96 from PixelgenTechnologies/fix/make-task-ext-cl…
fbdtemme Jun 17, 2024
f920319
Update citations
fbdtemme Jun 17, 2024
1cb639d
Update CHANGELOG
fbdtemme Jun 17, 2024
c320307
Update metromap to include layout step
fbdtemme Jun 18, 2024
20496c1
Update CHANGELOG
fbdtemme Jun 18, 2024
31e3dc5
Merge pull request #98 from PixelgenTechnologies/update-metromap
fbdtemme Jun 18, 2024
98238c8
fix: add layour docs to readme
ambarrio Jun 20, 2024
141642a
fix: changelog
ambarrio Jun 20, 2024
bfe93c4
fix: update output layout step
ambarrio Jun 20, 2024
0093847
Merge pull request #97 from PixelgenTechnologies/update-publication
fbdtemme Jun 24, 2024
12e795c
Merge pull request #99 from PixelgenTechnologies/chore/update-readme
ambarrio Jun 24, 2024
ca32765
Use R1/R2 suffixes in amplicon input fastq file renaming
fbdtemme Jun 27, 2024
9ea493a
Merge pull request #100 from PixelgenTechnologies/tweak-amplicon-rena…
fbdtemme Jun 27, 2024
dd34553
Fix validation issue when using panel_file instead of panel
fbdtemme Jun 27, 2024
dbaec78
Merge pull request #101 from PixelgenTechnologies/fix-panel-file
fbdtemme Jun 28, 2024
97bd99d
Restructure output directory
fbdtemme Jun 14, 2024
6ddf209
Update CHANGELOG
fbdtemme Jun 27, 2024
d16b018
Fix bad pattern for graph/*edgelist.parquet'
fbdtemme Jul 1, 2024
bead999
Use explicit return null in saveAs closures
fbdtemme Jul 1, 2024
d38af5c
Update docs/output.md
fbdtemme Jul 1, 2024
4f1de61
Make fastq file reference all caps
fbdtemme Jul 1, 2024
af797e5
Tweak graph stage output description
fbdtemme Jul 1, 2024
3516031
Tweak collapse output description
fbdtemme Jul 1, 2024
6ea4395
Merge pull request #102 from PixelgenTechnologies/restructure-output
fbdtemme Jul 1, 2024
115c87d
rate-diff is now default colocalization transform
johandahlberg Jul 8, 2024
d7d1e7e
Update the changelog
johandahlberg Jul 8, 2024
cbe7fc7
Empty commit to retrigger CI
johandahlberg Jul 8, 2024
51a9ec9
Merge pull request #103 from PixelgenTechnologies/make-rate-diff-defa…
johandahlberg Jul 8, 2024
faec0f4
Add colocalization_min_marker_count parameter
fbdtemme Jul 11, 2024
6acc037
Change default layout algorithm to wpmds_3d
fbdtemme Jul 11, 2024
a136ed0
Update pixelator container to 0.18 using override param
fbdtemme Jul 12, 2024
cadf011
Add v2 panel small test profile
fbdtemme Jul 12, 2024
96bd927
Update pixelator container to 0.18.1
fbdtemme Jul 15, 2024
e9e9035
Update CHANGELOG.md
fbdtemme Jul 15, 2024
b47a4bd
Remove unused parameter from test_v2.config
fbdtemme Jul 15, 2024
3790c92
Add `human-sc-immunology-spatial-proteomics-2` panel in docs
fbdtemme Jul 15, 2024
79ef889
Merge pull request #104 from PixelgenTechnologies/update-pixelator-0.…
fbdtemme Jul 15, 2024
2fd30b3
Bump version to 1.3.0
fbdtemme Jul 15, 2024
8c3a705
Merge pull request #105 from PixelgenTechnologies/release-1.3
fbdtemme Jul 15, 2024
ed4f50f
Apply suggestions from code review
fbdtemme Jul 15, 2024
b262018
Rename `test_v2` to `test_panel_v2` and align
fbdtemme Jul 15, 2024
3de2f54
Use `edge list` instead of `edgelist` in docs
fbdtemme Jul 15, 2024
12a2aa9
Output preqc html reports with `--save_all`
fbdtemme Jul 15, 2024
42c0653
Align code in nextflow.config
fbdtemme Jul 15, 2024
d701a59
Update test_panel_v2 usage comment
fbdtemme Jul 15, 2024
44fc31d
Fix tubemap icon alignment, add pipeline version
fbdtemme Jul 15, 2024
bc8526a
Apply suggestions from code review
fbdtemme Jul 16, 2024
ff3ab07
Update nextflow_schema.json
fbdtemme Jul 16, 2024
fdbd34d
Fix input paths to testdata in `test_panel_v2` config
fbdtemme Jul 16, 2024
3ffd206
Update to pixelator 0.18.2
fbdtemme Jul 16, 2024
a5247ba
Update release date in CHANGELOG
fbdtemme Jul 17, 2024
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26 changes: 26 additions & 0 deletions CHANGELOG.md
Original file line number Diff line number Diff line change
Expand Up @@ -3,6 +3,32 @@
The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).

## [[1.3.0](https://github.com/nf-core/pixelator/releases/tag/1.3.0)] - 2024-07-17

### Enhancements & fixes

- [[PR #97](https://github.com/nf-core/pixelator/pull/97)] - Update citations
- [[PR #96](https://github.com/nf-core/pixelator/pull/96)] - Make all ext.args assignments closures
- [[PR #98](https://github.com/nf-core/pixelator/pull/98)] - Update metromap to include layout step
- [[PR #99](https://github.com/nf-core/pixelator/pull/99)] - Update README to include layout step
- [[PR #100](https://github.com/nf-core/pixelator/pull/100)] - Use R1/R2 suffixes in amplicon input fastq file renaming
- [[PR #101](https://github.com/nf-core/pixelator/pull/101)] - Fix validation issue when using panel_file instead of panel
- [[PR #102](https://github.com/nf-core/pixelator/pull/101)] - Restructure output directory
- [[PR #103](https://github.com/nf-core/pixelator/pull/103)] - Make rate-diff the default transformation method when computing colocalization
- [[PR #104](https://github.com/nf-core/pixelator/pull/104)] - Update to pixelator 0.18.1
- [[PR #106](https://github.com/nf-core/pixelator/pull/106)] - Update to pixelator 0.18.2

### Software dependencies

| Dependency | Old version | New version |
| ----------- | ----------- | ----------- |
| `pixelator` | 0.17.1 | 0.18.2 |

> [!NOTE]
> Dependency has been **updated** if both old and new version information is present.
> Dependency has been **added** if just the new version information is present.
> Dependency has been **removed** if new version information isn't present.

## [[1.2.0](https://github.com/nf-core/pixelator/releases/tag/1.2.0)] - 2024-05-28

### Enhancements & fixes
Expand Down
4 changes: 2 additions & 2 deletions CITATIONS.md
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Expand Up @@ -10,9 +10,9 @@

## Pipeline tools

- [pixelator](https://doi.org/10.1101/2023.06.05.543770)
- [pixelator](https://doi.org/10.1038/s41592-024-02268-9)

> Karlsson, Filip, Tomasz Kallas, Divya Thiagarajan, Max Karlsson, Maud Schweitzer, Jose Fernandez Navarro, Louise Leijonancker, et al. “Molecular Pixelation: Single Cell Spatial Proteomics by Sequencing.” bioRxiv, June 8, 2023. https://doi.org/10.1101/2023.06.05.543770.
> Karlsson, F., Kallas, T., Thiagarajan, D. et al. “Molecular pixelation: spatial proteomics of single cells by sequencing.“ Nat Methods 21, 1044–1052 (2024). https://doi.org/10.1038/s41592-024-02268-9

- [cutadapt](http://dx.doi.org/10.14806/ej.17.1.200)

Expand Down
11 changes: 10 additions & 1 deletion README.md
Original file line number Diff line number Diff line change
Expand Up @@ -30,7 +30,8 @@ It takes a samplesheet as input and will process your data using `pixelator` to
5. Compute the components of the graph from the edge list in order to create putative cells ([`pixelator graph`](https://github.com/PixelgenTechnologies/pixelator))
6. Call and annotate cells ([`pixelator annotate`](https://github.com/PixelgenTechnologies/pixelator))
7. Analyze the cells for polarization and colocalization ([`pixelator analysis`](https://github.com/PixelgenTechnologies/pixelator))
8. Report generation ([`pixelator report`](https://github.com/PixelgenTechnologies/pixelator))
8. Generate 3D graph layouts for visualization of cells ([`pixelator layout`](https://github.com/PixelgenTechnologies/pixelator))
9. Report generation ([`pixelator report`](https://github.com/PixelgenTechnologies/pixelator))

> [!WARNING]
> Since Nextflow 23.07.0-edge, Nextflow no longer mounts the host's home directory when using Apptainer or Singularity.
Expand Down Expand Up @@ -101,3 +102,11 @@ You can cite the `nf-core` publication as follows:
> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.
>
> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).

You can cite the molecular pixelation technology as follows:

> **Molecular pixelation: spatial proteomics of single cells by sequencing.**
>
> Filip Karlsson, Tomasz Kallas, Divya Thiagarajan, Max Karlsson, Maud Schweitzer, Jose Fernandez Navarro, Louise Leijonancker, Sylvain Geny, Erik Pettersson, Jan Rhomberg-Kauert, Ludvig Larsson, Hanna van Ooijen, Stefan Petkov, Marcela González-Granillo, Jessica Bunz, Johan Dahlberg, Michele Simonetti, Prajakta Sathe, Petter Brodin, Alvaro Martinez Barrio & Simon Fredriksson
>
> _Nat Methods._ 2024 May 08. doi: [10.1038/s41592-024-02268-9](https://doi.org/10.1038/s41592-024-02268-9)
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