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@ryandward ryandward released this 01 Nov 20:44
· 3 commits to master since this release
v1.0
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Stable Release v1.0

Overview

This release marks the first stable version of the sgRNA Design Scripts for CRISPRi in Bacteria. The pipeline has undergone rigorous testing and is now considered stable for public use.

Features

  • Automated workflow for designing sgRNAs, encapsulated in build_sgrna_library.py.
  • Conda environment support with sgrna_design.yml for easy dependency management.
  • Integration with NCBI Entrez Direct Utilities for genome data retrieval.
  • Detailed documentation and quick-start guide.

Zenodo DOI

DOI

The stable release is archived on Zenodo for citation and long-term accessibility.

Usage Notes

  • Guides are suggested to have a small, positive offset, be on the antisense strand ('anti' in the 'transdir' column), and have a SPECIFICITY score of 39.
  • The repository is forked from the initial work by John S. Hawkins.
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