Stable Release v1.0
Overview
This release marks the first stable version of the sgRNA Design Scripts for CRISPRi in Bacteria. The pipeline has undergone rigorous testing and is now considered stable for public use.
Features
- Automated workflow for designing sgRNAs, encapsulated in
build_sgrna_library.py
. - Conda environment support with
sgrna_design.yml
for easy dependency management. - Integration with NCBI Entrez Direct Utilities for genome data retrieval.
- Detailed documentation and quick-start guide.
Zenodo DOI
The stable release is archived on Zenodo for citation and long-term accessibility.
Usage Notes
- Guides are suggested to have a small, positive offset, be on the antisense strand ('anti' in the 'transdir' column), and have a SPECIFICITY score of 39.
- The repository is forked from the initial work by John S. Hawkins.