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minor changes to project3
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Geert van Geest committed Mar 11, 2022
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1 change: 1 addition & 0 deletions .gitignore
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Expand Up @@ -10,3 +10,4 @@ workdir/
.parallel
.conda
.cpanm/
.bash_history
4 changes: 2 additions & 2 deletions docs/course_material/group_work/project3.md
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Expand Up @@ -75,7 +75,7 @@ You can do this for both the CLR reads and HiFi reads and compare the results.
```

**Before you run `prokka`**

The `conda` installation misses a perl module. Install it in the `assembly` environment like this:

```sh
Expand All @@ -90,7 +90,7 @@ You can do this for both the CLR reads and HiFi reads and compare the results.
* Check out the output. Where is the assembly? How is the quality? For that, check out `assembly_info.txt`.
* Check the completeness with `BUSCO`. Have a good look at the manual first. You can use automated lineage selecton by specifying `--auto-lineage-prok`. After you have run `BUSCO`, you can generate a nice completeness plot with `generate_plot.py`. You can check its usage with `generate_plot.py --help`.
* How is the completeness? Is this expected?
* Perform an annotation with `prokka`. Again, check the manual first. Have a look at for example the statistics in `PROKKA_[date].txt`, and for a nice table of annotated genes in `PROKKA_[data].tsv`.
* Perform an annotation with `prokka`. Again, check the manual first. After the run, have a look at for example the statistics in `PROKKA_[date].txt`. For a nice table of annotated genes have a look in `PROKKA_[data].tsv`.



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