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Deploy for release 0.1.1 from f639468
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rcannood committed May 3, 2023
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4 changes: 2 additions & 2 deletions CHANGELOG.md
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@@ -1,8 +1,8 @@
# utilities 0.1.1

* `mapping/process_10x_auto`: Allow grouping input fastqs per folder or via a custom regex statement.
* `mapping/process_smartseq2_auto`: Allow grouping input fastqs per folder or via a custom regex statement.

* `mapping/process_10x_auto`: Add more documentation to the arguments to clarify regex usage.
* `mapping/process_smartseq2_auto`: Add more documentation to the arguments to clarify regex usage.

# utilities 0.1.0

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9 changes: 4 additions & 5 deletions src/mapping/process_smartseq2/auto.vsh.yaml
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Expand Up @@ -11,11 +11,10 @@ functionality:
* Extract the sample id from the path name using the same `fastq_regex` and `sample_id_replacement`.
* Detect cell ids from the path name using the `fastq_regex` and `cell_id_replacement`.
* Write the list of samples as a yaml in the output directory.
* Map the reads using Cell Ranger
* Convert the Cell Ranger output to h5mu
* Remove ambient RNA with CellBender
* Remove cells with less than 100 genes or 1000 reads.
* Make the cell names unique
* Map the reads using Star
* Sort counts with samtools
* Convert to count table with HTSeq
* Compute QC metrics with MultiQC
* Output one h5mu file per sample
Concatenating the invididual h5mu files into one h5mu file is a separate pipeline.
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2 changes: 1 addition & 1 deletion target/docker/demux/spaceranger_mkfastq/.config.vsh.yaml
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Expand Up @@ -79,5 +79,5 @@ info:
output: "/home/runner/work/utilities/utilities/target/docker/demux/spaceranger_mkfastq"
executable: "/home/runner/work/utilities/utilities/target/docker/demux/spaceranger_mkfastq/spaceranger_mkfastq"
viash_version: "0.7.3"
git_commit: "f4bc99bf82a915a68541bcfd58b146064c8dba2b"
git_commit: "f639468c9d0ed115776e10d0f7a3330fff04ab20"
git_remote: "https://github.com/czbiohub/utilities"
4 changes: 2 additions & 2 deletions target/docker/demux/spaceranger_mkfastq/spaceranger_mkfastq
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Expand Up @@ -399,9 +399,9 @@ FROM ghcr.io/data-intuitive/spaceranger:latest
RUN :
LABEL org.opencontainers.image.description="Companion container for running component demux spaceranger_mkfastq"
LABEL org.opencontainers.image.created="2023-05-03T20:09:52Z"
LABEL org.opencontainers.image.created="2023-05-03T21:05:52Z"
LABEL org.opencontainers.image.source="https://github.com/czbiohub/utilities"
LABEL org.opencontainers.image.revision="f4bc99bf82a915a68541bcfd58b146064c8dba2b"
LABEL org.opencontainers.image.revision="f639468c9d0ed115776e10d0f7a3330fff04ab20"
LABEL org.opencontainers.image.version="0.1.1"
VIASHDOCKER
}
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2 changes: 1 addition & 1 deletion target/docker/mapping/spaceranger_count/.config.vsh.yaml
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Expand Up @@ -129,5 +129,5 @@ info:
output: "/home/runner/work/utilities/utilities/target/docker/mapping/spaceranger_count"
executable: "/home/runner/work/utilities/utilities/target/docker/mapping/spaceranger_count/spaceranger_count"
viash_version: "0.7.3"
git_commit: "f4bc99bf82a915a68541bcfd58b146064c8dba2b"
git_commit: "f639468c9d0ed115776e10d0f7a3330fff04ab20"
git_remote: "https://github.com/czbiohub/utilities"
4 changes: 2 additions & 2 deletions target/docker/mapping/spaceranger_count/spaceranger_count
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Expand Up @@ -423,9 +423,9 @@ FROM ghcr.io/data-intuitive/spaceranger:latest
RUN :
LABEL org.opencontainers.image.description="Companion container for running component mapping spaceranger_count"
LABEL org.opencontainers.image.created="2023-05-03T20:09:52Z"
LABEL org.opencontainers.image.created="2023-05-03T21:05:53Z"
LABEL org.opencontainers.image.source="https://github.com/czbiohub/utilities"
LABEL org.opencontainers.image.revision="f4bc99bf82a915a68541bcfd58b146064c8dba2b"
LABEL org.opencontainers.image.revision="f639468c9d0ed115776e10d0f7a3330fff04ab20"
LABEL org.opencontainers.image.version="0.1.1"
VIASHDOCKER
}
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Expand Up @@ -105,5 +105,5 @@ info:
output: "/home/runner/work/utilities/utilities/target/docker/multiomics/cellranger_arc_aggr"
executable: "/home/runner/work/utilities/utilities/target/docker/multiomics/cellranger_arc_aggr/cellranger_arc_aggr"
viash_version: "0.7.3"
git_commit: "f4bc99bf82a915a68541bcfd58b146064c8dba2b"
git_commit: "f639468c9d0ed115776e10d0f7a3330fff04ab20"
git_remote: "https://github.com/czbiohub/utilities"
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Expand Up @@ -418,9 +418,9 @@ RUN pip install --upgrade pip && \
pip install --upgrade --no-cache-dir "numpy" "pandas" "scanpy"
LABEL org.opencontainers.image.description="Companion container for running component multiomics cellranger_arc_aggr"
LABEL org.opencontainers.image.created="2023-05-03T20:09:52Z"
LABEL org.opencontainers.image.created="2023-05-03T21:05:53Z"
LABEL org.opencontainers.image.source="https://github.com/czbiohub/utilities"
LABEL org.opencontainers.image.revision="f4bc99bf82a915a68541bcfd58b146064c8dba2b"
LABEL org.opencontainers.image.revision="f639468c9d0ed115776e10d0f7a3330fff04ab20"
LABEL org.opencontainers.image.version="0.1.1"
VIASHDOCKER
}
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Expand Up @@ -97,5 +97,5 @@ info:
output: "/home/runner/work/utilities/utilities/target/docker/multiomics/cellranger_arc_count"
executable: "/home/runner/work/utilities/utilities/target/docker/multiomics/cellranger_arc_count/cellranger_arc_count"
viash_version: "0.7.3"
git_commit: "f4bc99bf82a915a68541bcfd58b146064c8dba2b"
git_commit: "f639468c9d0ed115776e10d0f7a3330fff04ab20"
git_remote: "https://github.com/czbiohub/utilities"
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Expand Up @@ -416,9 +416,9 @@ FROM ghcr.io/data-intuitive/cellranger_arc:2.0
RUN :
LABEL org.opencontainers.image.description="Companion container for running component multiomics cellranger_arc_count"
LABEL org.opencontainers.image.created="2023-05-03T20:09:52Z"
LABEL org.opencontainers.image.created="2023-05-03T21:05:53Z"
LABEL org.opencontainers.image.source="https://github.com/czbiohub/utilities"
LABEL org.opencontainers.image.revision="f4bc99bf82a915a68541bcfd58b146064c8dba2b"
LABEL org.opencontainers.image.revision="f639468c9d0ed115776e10d0f7a3330fff04ab20"
LABEL org.opencontainers.image.version="0.1.1"
VIASHDOCKER
}
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Expand Up @@ -95,5 +95,5 @@ info:
output: "/home/runner/work/utilities/utilities/target/docker/multiomics/cellranger_cite_seq"
executable: "/home/runner/work/utilities/utilities/target/docker/multiomics/cellranger_cite_seq/cellranger_cite_seq"
viash_version: "0.7.3"
git_commit: "f4bc99bf82a915a68541bcfd58b146064c8dba2b"
git_commit: "f639468c9d0ed115776e10d0f7a3330fff04ab20"
git_remote: "https://github.com/czbiohub/utilities"
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Expand Up @@ -409,9 +409,9 @@ FROM ghcr.io/data-intuitive/cellranger_arc:2.0
RUN :
LABEL org.opencontainers.image.description="Companion container for running component multiomics cellranger_cite_seq"
LABEL org.opencontainers.image.created="2023-05-03T20:09:53Z"
LABEL org.opencontainers.image.created="2023-05-03T21:05:54Z"
LABEL org.opencontainers.image.source="https://github.com/czbiohub/utilities"
LABEL org.opencontainers.image.revision="f4bc99bf82a915a68541bcfd58b146064c8dba2b"
LABEL org.opencontainers.image.revision="f639468c9d0ed115776e10d0f7a3330fff04ab20"
LABEL org.opencontainers.image.version="0.1.1"
VIASHDOCKER
}
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Expand Up @@ -122,5 +122,5 @@ info:
output: "/home/runner/work/utilities/utilities/target/docker/operations/create_runner_script"
executable: "/home/runner/work/utilities/utilities/target/docker/operations/create_runner_script/create_runner_script"
viash_version: "0.7.3"
git_commit: "f4bc99bf82a915a68541bcfd58b146064c8dba2b"
git_commit: "f639468c9d0ed115776e10d0f7a3330fff04ab20"
git_remote: "https://github.com/czbiohub/utilities"
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Expand Up @@ -426,9 +426,9 @@ FROM python:3.10
RUN :
LABEL org.opencontainers.image.description="Companion container for running component operations create_runner_script"
LABEL org.opencontainers.image.created="2023-05-03T20:09:52Z"
LABEL org.opencontainers.image.created="2023-05-03T21:05:52Z"
LABEL org.opencontainers.image.source="https://github.com/czbiohub/utilities"
LABEL org.opencontainers.image.revision="f4bc99bf82a915a68541bcfd58b146064c8dba2b"
LABEL org.opencontainers.image.revision="f639468c9d0ed115776e10d0f7a3330fff04ab20"
LABEL org.opencontainers.image.version="0.1.1"
VIASHDOCKER
}
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2 changes: 1 addition & 1 deletion target/native/demux/spaceranger_mkfastq/.config.vsh.yaml
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Expand Up @@ -79,5 +79,5 @@ info:
output: "/home/runner/work/utilities/utilities/target/native/demux/spaceranger_mkfastq"
executable: "/home/runner/work/utilities/utilities/target/native/demux/spaceranger_mkfastq/spaceranger_mkfastq"
viash_version: "0.7.3"
git_commit: "f4bc99bf82a915a68541bcfd58b146064c8dba2b"
git_commit: "f639468c9d0ed115776e10d0f7a3330fff04ab20"
git_remote: "https://github.com/czbiohub/utilities"
2 changes: 1 addition & 1 deletion target/native/mapping/spaceranger_count/.config.vsh.yaml
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Expand Up @@ -129,5 +129,5 @@ info:
output: "/home/runner/work/utilities/utilities/target/native/mapping/spaceranger_count"
executable: "/home/runner/work/utilities/utilities/target/native/mapping/spaceranger_count/spaceranger_count"
viash_version: "0.7.3"
git_commit: "f4bc99bf82a915a68541bcfd58b146064c8dba2b"
git_commit: "f639468c9d0ed115776e10d0f7a3330fff04ab20"
git_remote: "https://github.com/czbiohub/utilities"
Original file line number Diff line number Diff line change
Expand Up @@ -105,5 +105,5 @@ info:
output: "/home/runner/work/utilities/utilities/target/native/multiomics/cellranger_arc_aggr"
executable: "/home/runner/work/utilities/utilities/target/native/multiomics/cellranger_arc_aggr/cellranger_arc_aggr"
viash_version: "0.7.3"
git_commit: "f4bc99bf82a915a68541bcfd58b146064c8dba2b"
git_commit: "f639468c9d0ed115776e10d0f7a3330fff04ab20"
git_remote: "https://github.com/czbiohub/utilities"
Original file line number Diff line number Diff line change
Expand Up @@ -97,5 +97,5 @@ info:
output: "/home/runner/work/utilities/utilities/target/native/multiomics/cellranger_arc_count"
executable: "/home/runner/work/utilities/utilities/target/native/multiomics/cellranger_arc_count/cellranger_arc_count"
viash_version: "0.7.3"
git_commit: "f4bc99bf82a915a68541bcfd58b146064c8dba2b"
git_commit: "f639468c9d0ed115776e10d0f7a3330fff04ab20"
git_remote: "https://github.com/czbiohub/utilities"
Original file line number Diff line number Diff line change
Expand Up @@ -95,5 +95,5 @@ info:
output: "/home/runner/work/utilities/utilities/target/native/multiomics/cellranger_cite_seq"
executable: "/home/runner/work/utilities/utilities/target/native/multiomics/cellranger_cite_seq/cellranger_cite_seq"
viash_version: "0.7.3"
git_commit: "f4bc99bf82a915a68541bcfd58b146064c8dba2b"
git_commit: "f639468c9d0ed115776e10d0f7a3330fff04ab20"
git_remote: "https://github.com/czbiohub/utilities"
Original file line number Diff line number Diff line change
Expand Up @@ -122,5 +122,5 @@ info:
output: "/home/runner/work/utilities/utilities/target/native/operations/create_runner_script"
executable: "/home/runner/work/utilities/utilities/target/native/operations/create_runner_script/create_runner_script"
viash_version: "0.7.3"
git_commit: "f4bc99bf82a915a68541bcfd58b146064c8dba2b"
git_commit: "f639468c9d0ed115776e10d0f7a3330fff04ab20"
git_remote: "https://github.com/czbiohub/utilities"
2 changes: 1 addition & 1 deletion target/nextflow/demux/spaceranger_mkfastq/.config.vsh.yaml
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Expand Up @@ -79,5 +79,5 @@ info:
output: "/home/runner/work/utilities/utilities/target/nextflow/demux/spaceranger_mkfastq"
executable: "/home/runner/work/utilities/utilities/target/nextflow/demux/spaceranger_mkfastq/spaceranger_mkfastq"
viash_version: "0.7.3"
git_commit: "f4bc99bf82a915a68541bcfd58b146064c8dba2b"
git_commit: "f639468c9d0ed115776e10d0f7a3330fff04ab20"
git_remote: "https://github.com/czbiohub/utilities"
2 changes: 1 addition & 1 deletion target/nextflow/demux/spaceranger_mkfastq/main.nf
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Expand Up @@ -123,7 +123,7 @@ thisConfig = processConfig(jsonSlurper.parseText('''{
"config" : "/home/runner/work/utilities/utilities/src/demux/spaceranger_mkfastq/config.vsh.yaml",
"platform" : "nextflow",
"viash_version" : "0.7.3",
"git_commit" : "f4bc99bf82a915a68541bcfd58b146064c8dba2b",
"git_commit" : "f639468c9d0ed115776e10d0f7a3330fff04ab20",
"git_remote" : "https://github.com/czbiohub/utilities"
}
}'''))
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2 changes: 1 addition & 1 deletion target/nextflow/mapping/process_10x/.config.vsh.yaml
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Expand Up @@ -105,5 +105,5 @@ info:
output: "/home/runner/work/utilities/utilities/target/nextflow/mapping/process_10x"
executable: "/home/runner/work/utilities/utilities/target/nextflow/mapping/process_10x/process_10x"
viash_version: "0.7.3"
git_commit: "f4bc99bf82a915a68541bcfd58b146064c8dba2b"
git_commit: "f639468c9d0ed115776e10d0f7a3330fff04ab20"
git_remote: "https://github.com/czbiohub/utilities"
2 changes: 1 addition & 1 deletion target/nextflow/mapping/process_10x/main.nf
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Expand Up @@ -168,7 +168,7 @@ thisConfig = processConfig(jsonSlurper.parseText('''{
"config" : "/home/runner/work/utilities/utilities/src/mapping/process_10x/config.vsh.yaml",
"platform" : "nextflow",
"viash_version" : "0.7.3",
"git_commit" : "f4bc99bf82a915a68541bcfd58b146064c8dba2b",
"git_commit" : "f639468c9d0ed115776e10d0f7a3330fff04ab20",
"git_remote" : "https://github.com/czbiohub/utilities"
}
}'''))
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2 changes: 1 addition & 1 deletion target/nextflow/mapping/process_10x_auto/.config.vsh.yaml
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Expand Up @@ -150,5 +150,5 @@ info:
output: "/home/runner/work/utilities/utilities/target/nextflow/mapping/process_10x_auto"
executable: "/home/runner/work/utilities/utilities/target/nextflow/mapping/process_10x_auto/process_10x_auto"
viash_version: "0.7.3"
git_commit: "f4bc99bf82a915a68541bcfd58b146064c8dba2b"
git_commit: "f639468c9d0ed115776e10d0f7a3330fff04ab20"
git_remote: "https://github.com/czbiohub/utilities"
2 changes: 1 addition & 1 deletion target/nextflow/mapping/process_10x_auto/main.nf
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Expand Up @@ -208,7 +208,7 @@ thisConfig = processConfig(jsonSlurper.parseText('''{
"config" : "/home/runner/work/utilities/utilities/src/mapping/process_10x/auto.vsh.yaml",
"platform" : "nextflow",
"viash_version" : "0.7.3",
"git_commit" : "f4bc99bf82a915a68541bcfd58b146064c8dba2b",
"git_commit" : "f639468c9d0ed115776e10d0f7a3330fff04ab20",
"git_remote" : "https://github.com/czbiohub/utilities"
}
}'''))
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2 changes: 1 addition & 1 deletion target/nextflow/mapping/process_smartseq2/.config.vsh.yaml
Original file line number Diff line number Diff line change
Expand Up @@ -146,5 +146,5 @@ info:
output: "/home/runner/work/utilities/utilities/target/nextflow/mapping/process_smartseq2"
executable: "/home/runner/work/utilities/utilities/target/nextflow/mapping/process_smartseq2/process_smartseq2"
viash_version: "0.7.3"
git_commit: "f4bc99bf82a915a68541bcfd58b146064c8dba2b"
git_commit: "f639468c9d0ed115776e10d0f7a3330fff04ab20"
git_remote: "https://github.com/czbiohub/utilities"
2 changes: 1 addition & 1 deletion target/nextflow/mapping/process_smartseq2/main.nf
Original file line number Diff line number Diff line change
Expand Up @@ -216,7 +216,7 @@ thisConfig = processConfig(jsonSlurper.parseText('''{
"config" : "/home/runner/work/utilities/utilities/src/mapping/process_smartseq2/config.vsh.yaml",
"platform" : "nextflow",
"viash_version" : "0.7.3",
"git_commit" : "f4bc99bf82a915a68541bcfd58b146064c8dba2b",
"git_commit" : "f639468c9d0ed115776e10d0f7a3330fff04ab20",
"git_remote" : "https://github.com/czbiohub/utilities"
}
}'''))
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10 changes: 5 additions & 5 deletions target/nextflow/mapping/process_smartseq2_auto/.config.vsh.yaml
Original file line number Diff line number Diff line change
Expand Up @@ -153,10 +153,10 @@ functionality:
\ name using the same `fastq_regex` and `sample_id_replacement`.\n * Detect cell\
\ ids from the path name using the `fastq_regex` and `cell_id_replacement`.\n\
\ * Write the list of samples as a yaml in the output directory.\n * Map the\
\ reads using Cell Ranger\n * Convert the Cell Ranger output to h5mu\n * Remove\
\ ambient RNA with CellBender\n * Remove cells with less than 100 genes or 1000\
\ reads.\n * Make the cell names unique\n * Output one h5mu file per sample\n\
\nConcatenating the invididual h5mu files into one h5mu file is a separate pipeline.\n"
\ reads using Star\n * Sort counts with samtools\n * Convert to count table\
\ with HTSeq\n * Compute QC metrics with MultiQC\n * Output one h5mu file per\
\ sample\n\nConcatenating the invididual h5mu files into one h5mu file is a separate\
\ pipeline.\n"
status: "enabled"
set_wd_to_resources_dir: false
platforms:
Expand All @@ -178,5 +178,5 @@ info:
output: "/home/runner/work/utilities/utilities/target/nextflow/mapping/process_smartseq2_auto"
executable: "/home/runner/work/utilities/utilities/target/nextflow/mapping/process_smartseq2_auto/process_smartseq2_auto"
viash_version: "0.7.3"
git_commit: "f4bc99bf82a915a68541bcfd58b146064c8dba2b"
git_commit: "f639468c9d0ed115776e10d0f7a3330fff04ab20"
git_remote: "https://github.com/czbiohub/utilities"
4 changes: 2 additions & 2 deletions target/nextflow/mapping/process_smartseq2_auto/main.nf
Original file line number Diff line number Diff line change
Expand Up @@ -216,7 +216,7 @@ thisConfig = processConfig(jsonSlurper.parseText('''{
"entrypoint" : "auto"
}
],
"description" : "Map all SmartSeq2 Fastq files in a directory.\n\nSteps in the workflow:\n\n * Look for all `*.fastq.gz` files in the `input_dir` which\n match the regular expression in `fastq_regex`.\n * Extract the sample id from the path name using the same `fastq_regex` and `sample_id_replacement`.\n * Detect cell ids from the path name using the `fastq_regex` and `cell_id_replacement`.\n * Write the list of samples as a yaml in the output directory.\n * Map the reads using Cell Ranger\n * Convert the Cell Ranger output to h5mu\n * Remove ambient RNA with CellBender\n * Remove cells with less than 100 genes or 1000 reads.\n * Make the cell names unique\n * Output one h5mu file per sample\n\nConcatenating the invididual h5mu files into one h5mu file is a separate pipeline.\n",
"description" : "Map all SmartSeq2 Fastq files in a directory.\n\nSteps in the workflow:\n\n * Look for all `*.fastq.gz` files in the `input_dir` which\n match the regular expression in `fastq_regex`.\n * Extract the sample id from the path name using the same `fastq_regex` and `sample_id_replacement`.\n * Detect cell ids from the path name using the `fastq_regex` and `cell_id_replacement`.\n * Write the list of samples as a yaml in the output directory.\n * Map the reads using Star\n * Sort counts with samtools\n * Convert to count table with HTSeq\n * Compute QC metrics with MultiQC\n * Output one h5mu file per sample\n\nConcatenating the invididual h5mu files into one h5mu file is a separate pipeline.\n",
"status" : "enabled",
"set_wd_to_resources_dir" : false
},
Expand All @@ -242,7 +242,7 @@ thisConfig = processConfig(jsonSlurper.parseText('''{
"config" : "/home/runner/work/utilities/utilities/src/mapping/process_smartseq2/auto.vsh.yaml",
"platform" : "nextflow",
"viash_version" : "0.7.3",
"git_commit" : "f4bc99bf82a915a68541bcfd58b146064c8dba2b",
"git_commit" : "f639468c9d0ed115776e10d0f7a3330fff04ab20",
"git_remote" : "https://github.com/czbiohub/utilities"
}
}'''))
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Expand Up @@ -3,6 +3,6 @@ manifest {
mainScript = 'main.nf'
nextflowVersion = '!>=20.12.1-edge'
version = '0.1.1'
description = 'Map all SmartSeq2 Fastq files in a directory.\n\nSteps in the workflow:\n\n * Look for all `*.fastq.gz` files in the `input_dir` which\n match the regular expression in `fastq_regex`.\n * Extract the sample id from the path name using the same `fastq_regex` and `sample_id_replacement`.\n * Detect cell ids from the path name using the `fastq_regex` and `cell_id_replacement`.\n * Write the list of samples as a yaml in the output directory.\n * Map the reads using Cell Ranger\n * Convert the Cell Ranger output to h5mu\n * Remove ambient RNA with CellBender\n * Remove cells with less than 100 genes or 1000 reads.\n * Make the cell names unique\n * Output one h5mu file per sample\n\nConcatenating the invididual h5mu files into one h5mu file is a separate pipeline.\n'
description = 'Map all SmartSeq2 Fastq files in a directory.\n\nSteps in the workflow:\n\n * Look for all `*.fastq.gz` files in the `input_dir` which\n match the regular expression in `fastq_regex`.\n * Extract the sample id from the path name using the same `fastq_regex` and `sample_id_replacement`.\n * Detect cell ids from the path name using the `fastq_regex` and `cell_id_replacement`.\n * Write the list of samples as a yaml in the output directory.\n * Map the reads using Star\n * Sort counts with samtools\n * Convert to count table with HTSeq\n * Compute QC metrics with MultiQC\n * Output one h5mu file per sample\n\nConcatenating the invididual h5mu files into one h5mu file is a separate pipeline.\n'
author = 'Angela Pisco, Robrecht Cannoodt'
}
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