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VODKA

Viral Open-source DVG Key Algorithm

Usage

VODKA --genomeFA <input fasta> --queryInput <file of fastq/fasta> --outDir <output directory> \
--bt2Dir <bowtie2 directory> --webLogo <webLogo> [options]
  • --genomeFA <input fasta> : viral genome fasta file.

  • --queryInput <fastq/fastq> : a file with full path of fastq or fasta files for alignment. fastq/fasta files can be gzip'ed.

  • --outDir <output directory> : full path to output directory.

  • --bt2Dir <bowtie2 directory> : full path to bowtie2-2.2.9 directory. (where bowtie2 and bowtie2-build is located)

  • --webLogo <webLogo> : full path to webLogo.

  • options :

    • -bp_from_right <n> : <n> is number of bases from right. (default: 3000)
    • -h/--help : print this usage.

Requirements

  • bowtie2-2.2.9: Download bowtie2-2.2.9 from here.
  • webLogo: Download webLogo (version 3.5) from here.
  • RAM: 2.5G per fastq/fasta file (for reads longer than 150bp, 3G per fastq/fasta).