Significant Differences in Nucleotide and Peptide Features Between Chromosomes Suggesting Sequence Non Randomness Across Chromosomes.

Citation: Lim, GZK, Azmi, HH, Dolmatova, M, Ling, MHT. 2021. Significant Differences in Nucleotide and Peptide Features Between Chromosomes Suggesting Sequence Non-Randomness Across Chromosomes. Acta Scientific Microbiology 4(4): 23-28.

Link to [PDF].

Here is the permanent [PDF], [Supplementary Materials], and data set (ChromosomesFeatures.zip.001, ChromosomesFeatures.zip.002, and ChromosomesFeatures.zip.003) links to my archive.

Eukaryotic genomes are organized into multiple chromosomes. Several studies have suggested that chromosomes are organized functionally and spatially, indicative of selective pressure in chromosomal organization. However, question remains as to whether chromosomes of the same organism are significantly different based on nucleotide and peptide features. Here, we examine three eukaryotic species across kingdoms; animalia (Sarcophilus harrisii), plantae (Prunus dulcis), and SAR supergroup (Plasmodium falciparum); to identify whether chromosomes of the same organism are significantly different based on nucleotide and peptide features. Our results show that the average GC contents in coding sequences are significantly different (p-value ≤ 3.30E-09) to their chromosomal GC content in all 30 chromosomes across 3 organisms. Our results also show that 38 out of 45 (15 features by 3 organisms) the nucleotide and peptide features are significantly different (p-value ≤ 0.044) between chromosomes. These results imply the presence of selective pressure in chromosomal organization.