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other k mer resources
Kamil S. Jaron edited this page Sep 13, 2021
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- KMC: a faster and versatile k-mer counter
- KAT: k-mer counting and comparison tool, capable of comparing multiple libraries.
- khmer: probabilistic k-mer counting data structure, a compressible De Bruijn graph representation, De Bruijn graph partitioning, and digital normalization.
- Jellyfish: very easy to operate, but certainly not the fastest k-mer counter out there
- FASTK: Presumably the fastest counter, but it is unable to count repetitive kmers; using a workaround and modified version of genomescope called Genomescope.FK the genome size estimates are still in the right ballpark.
- GenomeScope - a kmer spectra analysis tool suited for polyploids as well
- tetramer - fitting pamaters to k-mer spectra of allotetraploids
- findGSE - an alternative model for fitting genome sizes
- KAT - KAT can plot the k-mer spectra with colouring by the frequency of the given k-mer in assembly, which is a very smart way how to inspect collapses of paralogs and duplicates.
- Merqury - Just like KAT but also made for trio data and coloring the assembly by the maternal and paternal contribution
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Rob Edwards' video introduction to concept of k-mers
- it's mostly in the context of genome assembly
- Bernardo Clavijo's introduction to k-mer counting
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A post about k-mers - this time for taxonomy!
- partially based on k-mers and sourmash
Introduction
k-mer spectra analysis
- 📖 Introduction to K-mer spectra analysis
- 📖 Basics of genome modeling
- ⚒ manual model fitting (for better understanding of the underlying model)
- ⚒ simple diploid
- ⚒ demonstrating the effect of sequencing error rate on k-mer coverage
- 📖 Common difficulties in characterisation of diploid genomes using k mer spectra analysis
- ⚒ low coverage (pitfall) - to be merged
- ⚒ very homozygous diploid
- ⚒ highly heterozygous diploid
- ⚒ Genome size of a repetitive genome (pitfall)
- ⚒ Wrong ploidy (pitfall)
- 📖 Characterization of polyploid genomes using k mer spectra analysis
- ⚒ Autotetraploid
- ⚒ Allotetraploid
- ⚒ Estimating ploidy (smudgeplot)
- 📖 Genome modeling as a quality control
- ⚒ Contamination (pitfall)
- ⚒ k-mers in an assembly (Mercury/KAT)
- 📖 Analysing genome skimming data
Separation of chromosomes
- 📖Separate sub-genomes of an allopolyploid
- 📖Separating chromosomes by comparison of sequencing libraries
Species assignment using short k-mers
Others