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other k mer resources

Kamil S. Jaron edited this page Sep 13, 2021 · 7 revisions

Software

K-mer counters

  • KMC: a faster and versatile k-mer counter
  • KAT: k-mer counting and comparison tool, capable of comparing multiple libraries.
  • khmer: probabilistic k-mer counting data structure, a compressible De Bruijn graph representation, De Bruijn graph partitioning, and digital normalization.
  • Jellyfish: very easy to operate, but certainly not the fastest k-mer counter out there
  • FASTK: Presumably the fastest counter, but it is unable to count repetitive kmers; using a workaround and modified version of genomescope called Genomescope.FK the genome size estimates are still in the right ballpark.

Kmer spectra analysis tools

  • GenomeScope - a kmer spectra analysis tool suited for polyploids as well
  • tetramer - fitting pamaters to k-mer spectra of allotetraploids
  • findGSE - an alternative model for fitting genome sizes

QC of genome assembly

  • KAT - KAT can plot the k-mer spectra with colouring by the frequency of the given k-mer in assembly, which is a very smart way how to inspect collapses of paralogs and duplicates.
  • Merqury - Just like KAT but also made for trio data and coloring the assembly by the maternal and paternal contribution

Learning materials

General

Taxonomy

Table of content

Introduction

k-mer spectra analysis

Separation of chromosomes

Species assignment using short k-mers

Others

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